I'm going to analyse plasma samples that contain a lot of fibrin. It is necessary to centrifuge the samples to be able to pipette the plasma. After the samples are centrifuged I can't vortex them because then I mix everything again and it wont be possible to pipette. Now I wonder whether it is necessary to vortex them before I centrifuge them. I don't think so because during centrifugation particles will anyway be separated by density. Or am I wrong? And in that case why? Appreciate your thoughts!

  • $\begingroup$ Are you talking about plasma or serum? Did you already separate either from blood sample? You need to include these specific info in your question. $\endgroup$ – Mathew Mahindaratne Feb 10 at 17:04
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    $\begingroup$ I'm voting to close this question as off-topic because it doesn't seem to be about chemistry. $\endgroup$ – Mithoron Feb 10 at 17:55
  • $\begingroup$ @Mathew Mahindaratne it is plasma and yes it is allready separated from erytrocytes. Sorry for leaving out information! $\endgroup$ – Anna Feb 10 at 19:50
  • $\begingroup$ @Mithoron It is about how chemicals separate, do you know a more suitable forum for this type of question? $\endgroup$ – Anna Feb 10 at 20:00
  • $\begingroup$ Visible solid fiber particle (e.g., fibrin) will separate in higher speed centrifugation by gravity (not really by density). If your analysis is based on density gradient, you should use ultra-centrifuge (rpm is about $1.0 \times 10^5$ or higher). $\endgroup$ – Mathew Mahindaratne Feb 10 at 20:00