I have pepsin powder which is now hydrated due to humidity. I want to convert it back to the powder form. How to do it?

I don't have access to a freeze-dryer . I prefer any cheap procedure other than freeze-dryer .

  • $\begingroup$ What is "cheap"? A lab scale vacuum tray dryer is probably ~1,000 USD cheaper than a lab scale freeze dryer. $\endgroup$ – J. Ari Nov 11 '19 at 17:50
  • $\begingroup$ An old paper: doi.org/10.1021/ac60329a047 describes the cheap way of dehydrating serum. I guess that the low temperature, achieved with dry ice in acetone, prevents denaturation as the solvent is removed. The rest can apparently be done in a vacuum desiccator. As your sample is not solvated, but just wet, I would try the method on a small amount and check if reactivity is preserved $\endgroup$ – The_Vinz Nov 12 '19 at 0:25
  • $\begingroup$ Try using a desiccator with a desiccant such as CaCl2 (cheap) or another -- see en.wikipedia.org/wiki/List_of_desiccants. $\endgroup$ – DrMoishe Pippik Nov 12 '19 at 1:28
  • $\begingroup$ I accidently add some water in it... 20ml water in 100gm pepsin powder... $\endgroup$ – Esdras Nov 13 '19 at 20:55
  • $\begingroup$ Do you need it for its enzymatic property or as nutrient? $\endgroup$ – Karsten Theis Dec 17 '19 at 15:51

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