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Im trying to calculate ion strength and pH of solution.

Components of the solution: 47 ul of 1000 pmol DNA in Elution Buffer (10 mM Tris-HCl pH 8.5) was bound to 3 ul of 10 µM YoYo1 (flourescent dye) in DMSO and was mixed with 50 ul of Tris-EDTA buffer (100 mM Tris-HCl, 0.01 M EDTA pH 8.0).

About calculation of ion strength, Im confused what Ions are in the solution too. Tris-HCl => Tris-H + Cl- ? EDTA => Mg2+ + Ca2+?? DMSO => ??

Furthermore, how can I calculate it?

I think the pH range is about 8.0~8.5, because of two buffers. Is there any method for calculating more exact pH value?

Thank you so much for your answers!

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  • $\begingroup$ Why do you suppose TrisHCl EDTA buffer contains Ca/Mg ? $\endgroup$ – Poutnik Aug 15 at 6:07