1
$\begingroup$

I want to know if someone has supporting tips or solutions to my problem: In biotechnology, fermentations are used to grow microorganisms. These need a specific media/broth in which temperature, pH, substrate and many other factors conclude to an optimum. Only if the surrounding broth is in optimal condition for the individual microorganism, the microorganism will grow as best as possible.

In my media the broth is buffered with $[\ce{HA}] = \ce{H2PO4^-}$ and $[\ce{A-}] = \ce{HPO4^2-}$.

While the fermentation is running the pH is corrected by $2\ M\ \ce{NaOH}$ which is pumped into the broth. Via balances, the weight is recorded and the input can be calculated.

I know the initial concentrations of my buffer solutions and the concentration of the base that is dropped into at every minute of record.

How can I calcute the pH for an individual timepoint in my aqueous fermentation? My tries via the Henderson-Hasselbach-formula did not work so far... (Neglecting other surroundings such as stirring, the other media components, the biomass' metabolites, temperature influence on the pKs, etc.),

Thanks in advance!

Improve this question
$\endgroup$
2
  • $\begingroup$ I guess you are aiming to control the $p^H$. Are pH probes + a feedback/feedforward control system not possible? $\endgroup$
    – Eashaan Godbole
    Jul 16 '19 at 14:17
  • $\begingroup$ I need to calculate the pH at different times troughout the process to be able to implement a pH-model on a calculation/formula. It should be used to compare it to the probes and control system.Furthermore to the model made from probes + feedback/feedforward control system. $\endgroup$
    – Capt.Krusty
    Jul 16 '19 at 16:39
2
$\begingroup$

The pH is not corrected mathematically, it is stabilized chemically by addition of NaOH when the pH sensors get out of range. Your pH is controlled to be constant over the fermentation period (+/- a little bit).

The acid produced by the fermentation is neutralized by addition of NaOH to keep the pH constant. What you can calculate from the data you get is the rate of acid production. The moles of acid produced will equal the number of moles of NaOH added, to a good approximation. The pKa of H2PO4- is 7.2 and the pKa of acetic acid (a typical carboxylic acid) is 4.76, so the acid produced will be "strong" - i.e., fairly acidic in your medium, which appears to be aimed for about pH = 7 - and the NaOH is strongly basic, so they will balance out (approximately).

Interestingly, sodium acetate has a pH range of 8.5 - 9.9 in real life, according to SDS. Calculation is so much more pleasant, where you can calculate pH to three decimals and feel good about it (until you test it in real life and have to calibrate your meter, etc, etc.)

Improve this answer
$\endgroup$
2
  • $\begingroup$ Could you recommend literature on this specific topic? Such as implementing a control system for fermentations on basis of a pH probe. If the pH is corresponding to the acid concentration of the microorganism, it is responing to biomass itself and can therefore be used for prediction models. $\endgroup$
    – Capt.Krusty
    Jul 18 '19 at 10:14
  • $\begingroup$ Google brings up a lot of fermentation control system literature - but it seems to be mostly for controlling beer and wine. Major industries, of course! But maybe the info on one of these systems (before you buy!) would give you some ideas. In addition to the pH measurement, you could consider measuring temperature, O2 uptake (a little more difficult, but very interesting), CO2 production, conductivity, viscosity. Some of these parameters would just be measured for your model, and some could be controlled to eliminate their variability. $\endgroup$
    – James Gaidis
    Jul 18 '19 at 13:14

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.