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In my experiment I am passing a solution through an adsorption column, collecting the eluate, then passing the eluate back through the same column.

Is there a concise term which describes this process?

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  • $\begingroup$ reapply the eluate to the column? $\endgroup$ – Karsten Theis Jun 10 '19 at 19:57
  • $\begingroup$ Why are you doing this? $\endgroup$ – Karl Jun 10 '19 at 22:00
  • $\begingroup$ @Karl The solution effectively is influent, and the adsorption column removes a pollutant from it. The eluate (i.e. effluent), when passed back through the same column, further decreases the pollutant's concentration. Therefore I'm trying to find a term to describe the process. $\endgroup$ – George Tian Jun 11 '19 at 9:04
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This process is similar to re-cycling chromatography or recycling HPLC. In a more strict sense, it does not do any good, at least theoretically, if you keep on recycling all the eluate and transfer it back to the column.

What makes more sense is that you collect fractions and choose certain fractions for recycling.

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  • $\begingroup$ Indeed. It is like to perform multiple chromatography columns. Differently phrased and at least changing the second column is not too unusual. $\endgroup$ – Alchimista Jun 11 '19 at 11:11

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