I would like to use a microplate reader to measure the absorbance of dissolved organic matter (DOM), but the measured values are close to the lower detection limit. To overcome this, I thought of deep well plates as the pathlength is longer then. Nevertheless, I have not found any deep well plates with flat bottoms, which might be best for absorbance measurements. I want to avoid measuring in a photometer with 5 cm pathlength because the available amount of sample is limited and the microplate would need much less.
Does anybody have experience with similar problems?
Can anyone suggest a suitable microplate?
What is your opinion on the influence of the material?