1
$\begingroup$

I am using a smaller diameter column than my previous one keeping all other factors such as stationary phase, solvent, solvent gradient constant. (Obviously, flow rate will be less in smaller column). I would like to know will changing the diameter affect the retention time of my target?

I searched and got results like this and this But they do not mention about the effect of column diameter on retention time. Moreover, I am weak in understanding mathematical equations.

Improve this question
$\endgroup$
1
  • $\begingroup$ I do not see the way how it could directly affect the retention times. But it may affect them indirectly, e.g by affecting the column packing, or the provider using different production process. It may slightly affect HETP via column/particle diameter ratio. $\endgroup$
    – Poutnik
    May 27, 2019 at 11:03

1 Answer 1

Reset to default
1
$\begingroup$

The key idea is that when you change column diameter, you keep the superficial linear velocity the same. For example if I have an analyte which elutes on a 10 cm x 4.6 mm i.d column at 7 minutes at 1 mL/min. Now we change the diameter to 3 mm, we have to use a flow rate of 0.425 mL/min to keep the retention time the same. Now we wish reduce the diameter further, say 2.1 mm i.d., now you should use 0.21 mL/min so that your analyte elutes at 7 minutes.

Search the keyword: superficial linear velocity, HPLC

Improve this answer
$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.