I am currently conducting a series of experiments in which I am studying the transport of polystyrene nanoparticles (PNPs) through sand and soil. This is done in sand/soil columns, into which I am injecting the PNPs solution using a peristaltic pump. The PNPs are fluorescent, so I am using a fluorimeter for detection. During the experiment, I am collecting the fluid that is leaving the column in a fraction collector, and as a result I am constructing a breakthrough curve that is supposed to show me the behavior of the PNPs compared to an inert tracer (bromide) that is also found in the inlet solution.
In order to construct the breakthrough curve, I need to prepare a calibration curve that will allow me to convert the intensity of the collected fractions into concentrations. For the calibration curve I am preparing several samples with known concentrations.
For preparation of the calibration curve, I am choosing the wavelength that gives me the max intensity in each sample's emission spectrum, and then for the breakthrough curve I am taking the intensities in that same wavelength from each fraction for the breakthrough curve. In the example below it is 635 nm.
The X-axis range is the same: 575-700 nm. I need to choose a certain wavelength and take the data pertaining to it.
Can I actually use these intensities, or would it be wrong because at 635 nm I don't have a max point?