I need to make a citrate-sodium citrate buffer at 0.5 M concentration and pH 4.5. I calculated the molarities of the citrate and disodium citrate based on the Henderson Hasselbalch equation, using a pKa of 4.76 . To cross-check my calculations, I tried a couple of online calculators, but the results are way off from mine. I think the formulae should change based on where I use monosodium, disodium or trisodum citrate. What is the right way to go about making this calculation?

  • $\begingroup$ The relevant pKa for the trisodium citrate/disodium hydrogen citrate pair is 6.40. $\endgroup$ – Karsten Theis Jan 15 at 14:54

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