1
$\begingroup$

It seems that most HPLC-MS/MS methods used for quantitation of catecholamines (epinephrine, norepinephrine and dopamine, specifically) operate at low pH (commonly 3.0), and therefore demand the addition of ion pairing reagents if a C18 column is to be employed. From my understand, these conditions are used to improve catecholamine stability, and avoid formation of quinones by oxidation. Fair.

My question is; why are high pH solvents not used (> pKa + 2.0, so pH ~ 10.5), with the addition of antioxidizers, such as EDTA? I understand that this would require MS operation in negative ion mode, but should circumvent need for ion pairing reagents. Might this be possible? Has this been attempted?

$\endgroup$
  • $\begingroup$ As an update - it seems that oxidation of the catechol group is slow (Bonhomme et al, 1990; sciencedirect.com/science/article/pii/…), so perhaps a reducing agent is unnecessary? Hoping someone can provide some insight.. $\endgroup$ – JK.Robertson Aug 27 '18 at 14:34

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.