The chromatography tag has no wiki summary.
2
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1answer
146 views
Why should I add lithium bromide to my GPC eluent?
I'm running gel permeation chromatography (GPC) of a polar, uncharged polymer (acrylamide-based) in a polar solvent (dimethylformamide). The literature recommends adding a salt to the eluent; for my ...
1
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0answers
59 views
Calculating laser wavelength/power to cause emission of light in a gas?
It's been a long, long time since I've had to worry about electron excitation levels and band gaps. Please forgive (and correct!) my terminology and misconceptions.
I've become interested in ...
6
votes
1answer
121 views
How to translate the mobile phase from thin layer chromatography to column?
I recently was told that once you find a good solvent mixture for separation on thin layer chromatography (TLC), you should only use 10% of the more polar solvent (for silica) when you run your ...
5
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1answer
188 views
What is the behavior of a Zwitterion during ion exchange chromatography?
We're interested in purifying a protein that has an azide moiety using ion exchange chromatography. Due to its unique structure, the moiety is a Zwitterion with a novel chemical behavior. My question ...
3
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2answers
562 views
Gas chromatography/mass spectrometry: Can I use peak ratios and molecular mass to derive quantity?
Can derive the quantity of a substance from a GC/MS report if I know:
the ratio to another substance in the data,
the quantity of that second substance, and
the molecular mass of both substances.
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