The scientific study of chemical processes in living organisms, including, but not limited to, living matter.

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14 views

Carbonic acid-hydrogencarbonate buffer system pH calculation?

I am learning about the pH of buffer solutions, and am slightly confused by my text book's calculation. For the blood's buffer system of carbonic acid and hydrogencarbonate ions as the conjugate case, ...
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0answers
10 views

Microorganism preventative solution safe for using on a roof and will last in the sun

Information is scarce in the roof cleaning industry and the big guys are making a killing selling chemicals that are harmful to the person using it and everything around the home. I would like to find ...
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2answers
40 views

Pathway from Sucrose to Cholesterol

I was reading Linus Pauling's book How to Live Longer and Feel Better, and in various places in the book he discusses a correlation between increased sucrose intake and increased blood plasma ...
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0answers
28 views

Identify “decoupling sites” in Glucose Oxydase reaction

Given the following coupled reactions: $$\ce{Glucose + O2 + H2O + Glucose~Oxydase -> Gluconic~Acid + H2O2}$$ $$\ce{H2O2 + TMB(star) -> TMB}$$ Identify: A potential reagent or product that ...
2
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1answer
21 views

How do transmembrane protein alpha helixes bind to the membrane?

I would post this in biology but as the question is biochemical in nature I decided this would be a more appropriate platform. What I'm asking is how exactly does a transmembrane protein bind to the ...
3
votes
1answer
67 views

How do I know that an acid and conjugate base are present in equimolar amounts?

Acetylsalicylic acid is a molecule in several popular drugs such as Magnyl and aspirin. Its pKa is 3.5, and I'm about to calculate the pH of a 0.010 M solution of acetylsalicylic acid. I've read up ...
3
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1answer
36 views

Calculating charge of amino acids (am I doing it right?)

Please consider this picture of a peptide chain: I've done some examining on the different amino acids and concluded that the chain is as follows: Lys-Leu-Gly-Ser-Citrullin (variant of arginine). ...
1
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1answer
13 views

Determination of a chemical compound in a non-homogeneous sample

I want to know if it is possible to determine a chemical compound in a non-homogeneous sample. I am asking this because we are aiming to create a device that will detect histamine level in fish ...
2
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1answer
12 views

kinetic and thermodynamic parameters of biodiesel production from lard

I want to know if someone has experimental results and calculations of the kinetic and thermodynamic parameters of Base Catalyzed Transesterification of pig fat, if not which is the method used to ...
2
votes
1answer
50 views

The meaning of different parts in the naming of glucose

There are two main types of glucose, $\alpha$-D-(+)-glucose and $\beta$-D-(+)-glucose. Can anyone please explain what is $\alpha$,D or (+)? So (maybe by permutation and combination), how many types ...
1
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3answers
33 views

Biochem Book age and suggestions

I'm taking chem classes, and am interested in learning a some biochem on the side, on my own. I've bee looking for a decent book, though I'm on a bit of a tight budget so not brand shiny new ones ...
4
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2answers
188 views

How to buy 1g of enzyme that is sold in units/mg?

I am trying to buy 1g of horseradish peroxidase. On sigma-aldrich, it is sold as units/mg. I am confused as to what this unit is. It comes in 50-150, 150-250, and 250-330 u/mg. The way I currently ...
2
votes
2answers
30 views

Rate constant and Michaelis Menten

Please consider the following figure of a concentration over time (msec) diagram with variables for an enzyme, a substrate bound enzyme and a product. Recall that $\ce{E + S \leftrightharpoons ES ...
1
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1answer
32 views

Why does Ammonium sulfate cause proteins to precipitate?

I'm trying to understand an observed reaction from a chem practical: You have a solution of ammonium sulfate, which you add to a bovine serum albumin solution. The proteins precipitate, and the ...
2
votes
1answer
31 views

Why don't ions have one equilibrium potential? (Nernst equation)

I know that equilibrium potentials are dependent on the ratio of ion concentrations inside and outside of the cell and temperature. I also know that the equilibrium potential is reached when there is ...
1
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1answer
42 views

Hydrolysing large molecules to reveal amino acids

Consider Oxytocin: I am asked to hydrolyse Oxytocin and reveal 5 amino acids which are the result of this hydrolysis. I must admit I have no idea where to begin. I know that generally amino acids ...
0
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1answer
31 views

What is a triglyceride?

I'm confused on what a triglyceride is, from what my text book it says its a type of gylercide, then from a website it said Glycerides can be subdivided into two categories. The first group, the ...
3
votes
1answer
99 views

Is it possible to chemically treat grass to make it digestible?

Is it possible to chemically treat grass after "harvesting" it to make it digestible by humans? I know we can "eat" grass, but we can't break it down in our stomachs. If there is some chemical ...
2
votes
3answers
38 views

Why and when is lysine charged

I'm reading about Lysine and doing some calculations on it's charge under various $\ce{pH}$. Depending on the source, Lysine is usually stated as a charged amino acid with a $\ce{NH3+}$ group. ...
2
votes
1answer
41 views

How to rationalise the protonation state of amino acids

I am learning about amino acids in various $\ce{pH}$ environments and how their groups are protonated or deprotonated in these environments. I don't need to mention a specific acid for my question, I ...
2
votes
2answers
173 views

Chirality on Propranolol

I'm a bit confused when asked to specify the chiral carbons on this sketch of Propranolol as I'm not sure how the $\ce{H3C}$ and $\ce{CH3}$ play in if they are reversed in the name like that...Does ...
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0answers
15 views

Which solvent is good for using SU-8 particle in Optical tweezers?

I'm studying Optical tweezers. I've used Polystyrene for samples. Nowadays, I've got SU-8 pillar. I wanna use that for sample, but its density is heavier than water. I can't use that. So I wanna know ...
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1answer
36 views

Which chemicals upregulate Atoh1? [closed]

Atoh1 has been shown to have cancer fighting properties. I'd like to increase the notch pathway to make it more active. How can I increase the expression of Atoh1 with a vitamin or biochemical ...
0
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0answers
36 views

Aluminium in Active Carbon Water Filters

So I saw on article stating concerns about eating active carbon due to high levels of aluminium found, would this have implications with active carbon water filters? Or would the aluminium not get ...
0
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0answers
33 views

How could click reaction take place at low concentration?

I have DBCO-functionalized resin, and the DBCO group is validated with FAM-azide. But I cannot use this resin to react with FAM-Azide-labled primer, which has very low concentration (20 μM, it is ...
0
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1answer
20 views

What is the Rate of Reaction of Acetaldehyde Dehydrogenase from Saccharomyces Cerevisiae?

I am working on a biochemistry project, and I need to know some information about reaction rates. What is the Rate of Reaction of Acetaldehyde Dehydrogenase from Saccharomyces Cerevisiae?
2
votes
1answer
56 views

Why and how does lycopene absorbance increase with heating?

Lycopene, which is a carotenoid and a phytochemical is the main reason for why tomatoes are radio-protective. Source 1 . Source 2 Let's cut to the chase: I was wondering if this claim of The ...
4
votes
1answer
40 views

What happens to the NADH produced by lactate oxidation?

I first want to note that I'm a layman attempting to gain an understanding of metabolism by reading resources on the internet with only a little understanding of chemistry. I've hit a snag: Oxidation ...
6
votes
1answer
52 views

Is Acrylamide carcinogenic? Why?

Recently concerned with health effects by common chemicals existent in food I've been rather busy reading article after article; and just an interesting one came around: Acrylamide (or acrylic ...
5
votes
1answer
78 views

Finding specific activity of an enzyme from Km values for a reversible enzyme reaction

I have an enzymatic equation in the form: $$E + S\xleftrightarrow[k_{-1} = 2.6\text{ x }10^{-2} \text{s}^{-1}]{k_1 = 3 \text{ x }10^8 \text{M}^{-1}\text{s}^{-1}} EX \xleftrightarrow[k_{-2} = ...
4
votes
1answer
526 views

CO poisoning - What I've been taught is a hoax?

In elementary school, I happened to ask my teacher: What is $\ce{CO}$ they talk about these days? I was then taught that Carbon monoxide "competes" with the oxygen in our blood (we were taught ...
4
votes
1answer
30 views

What is the mechanism by which sugars glycate proteins?

I was looking at why it's ok for glucose to circulate through the blood while fructose is prevented from doing so by the liver. Fructose glycates proteins more readily than glucose. This is the ...
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1answer
56 views

I have 100mg of a proteinase K lyophilized powder and I need to make it to a working concentration of 25mg/mL

So I know that I need to add $4~mL$ of solution to this powder, which will give me a concentration of the enzyme at $25\frac{mg}{mL}$. What I do not understand is how to decide how much of each of ...
5
votes
1answer
140 views

Why is the pKa (COOH) of alanine and glycine similar but the pKa (NH_3) different?

Alanine is heavier with a ($\ce{CH_3}$) than glycine which has an ($\ce{H}$). What makes them have different $pK_{a}(\ce{NH_3})(pKa: Aly=9.69 , Gly=9.60)$ but similar $pK_{a}(\ce{COOH})(pKa=2.34) $ ...
0
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3answers
60 views

How does photosynthesis work?

I wonder how photosynthesis works. I read the Wikipedia page, but I'm still confused. My main gap of understanding is - I assume - : I know a photon changes the velocity of an electron. But I do not ...
2
votes
1answer
39 views

Do molecules with carboxyl groups have properties of carboxyl's sub-functional-groups?

We're learning about functional groups of different organic molecules, and there are some I'm confused about: carboxyl, carbonyl, and hydroxyl. I was looking online but I couldn't find a direct answer ...
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3answers
285 views

Why is the units of kcat 1/s?

I understand that $k_\text{cat}$ measures the turnover number of an enzyme. This measure is therefore a quantity of molecule conversions per unit of time. I suspect that my problem is more that of a ...
3
votes
1answer
65 views

what would happen if you got a full package of wet silica gel on your hand after it tore open? [closed]

I accidently opened a bag of silica gel and got it on my hand what will happen to my hand because now it kinda feels weird.
4
votes
3answers
77 views

Can a single monomer be a macromolecule? If so, when?

In our science class, we're learning about the four fundamental macromolecules in every living organism: carbohydrates, proteins, lipids, and nucleic acids. Our class definition of a macromolecule ...
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0answers
59 views

Resonance effect

I would need to know if there's a mesomeric effect in urea and L-citrullina molecules . Could anybody help me with this? I mean are this molecule both hybrids of resonance ? Wich are they resonance ...
0
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1answer
58 views

How does UV affect skin colors in dark-skinned people?

Skin color is one of the things one would rather not ask anything about! Only in humans, it can vary from very dark brown to pale pink. In darker-skinned people, the color is mainly due to melanin, ...
2
votes
1answer
85 views

How does Melatonin put you to sleep? How is this compound radio-protective?

Melatonin (IUPAC semi-formal name: N-acetyl-5-methoxytryptamine) is a hormone produced in humans, animals and some of plants. It's mainly famous for being the drug suggested to people with sleeping ...
2
votes
1answer
91 views

What compounds exist in the bitter peel of specific fruits?

Actually, my original question was: What makes a pith of pomegranate so bitter? Then I decided to be more generic. You'll hardly find someone that consumes pomegranate and doesn't nag about the bitter ...
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1answer
38 views

Aspartame poisoning: Can it modify aminoacids?

I just accidentally closed a webpage (and did not find it later) which described that somehow, Aspartame poisoning victims had faults in the vital proteins of their body due to the reaction of ...
15
votes
3answers
1k views

Why is methanol toxic?

There are two points of view for the answer of this question: The biological view, the only one that I faced during my research, states that since it can trigger perilous conditions like metabolic ...
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0answers
26 views

Finding the thermodynamics of protein unfolding from temperature and absorbance using fluorescence spectroscopy?

I recently did an experiment on protein unfolding using a Perkin Elmer fluorescence spectrometer. The protein was $\alpha$-chymotrypsin. I was given this paper for my procedure. I am now trying to ...
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7 views

Ethanol in gas & impact to old plastic motorcycle gas tanks

I recently began refurbishing a 1980 Suzuki dirt bike with a plastic gas tank One week after filling the tank with gas containing ethanol the tank developed a 5 inch long split. Is this caused by the ...
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0answers
57 views

Can we repair a dead body?

The following passage has been extracted from the book "Organic Chemistry-10th edition by Solomon and Frhyle": "The growth of living things from microbes to elephants rests on organic reactions, ...
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1answer
50 views

What is the Chemistry behind “good” and “bad” cholesterol & their relation to Cis/Trans fatty acids?

I have searched Google for this but cannot seem to find an explanation. My question is as follows - what is the Chemistry/Biochemistry behind cis and trans fatty acids resulting in "Bad" LDL and ...
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0answers
18 views

Can I break starch down into glucose units?

I was wondering if starch can be broken up into individual glucose units. Because on a site I saw, it said that through acid hydrolysis starch can be broken up into amylose and amylopectin. However, ...